rabbit calretinin calb2  (Novus Biologicals)

Journal: BMC Cancer

Article Title: Uterine tumors with sex cord-like elements: a clinicopathologic study of 19 cases

doi: 10.1186/s12885-026-15591-5

Figure Lengend Snippet: Calretinin, α-inhibin, Melan A and steroidogenic factor-1 (SF-1) expression in uterine tumor resembling ovarian sex cord tumor (UTROSCT) and adenosarcoma (AS). A Diffuse calretinin expression in UTROSCT; ( B ) Dot-like α-inhibin expression in UTROSCT; ( C ) Polygonal cells in UTROSCT showed Melan A positive; ( D ) SF-1 expression in UTROSCT; ( E - H ) Calretinin, α-inhibin, Melan A and SF-1 expression in AS. (×200)

Article Snippet: Calretinin , Polyclonal , Zhongshan Golden Bridge Biotechnology LLC , Beijing , China , DAKO.

Techniques: Expressing

Journal: Brain research

Article Title: Detailed neuronal distribution of GPR3 and its co-expression with EF-hand calcium-binding proteins in the mouse central nervous system.

doi: 10.1016/j.brainres.2020.147166

Figure Lengend Snippet: Fig. 2. Distribution and characterization of GPR3-positive neurons in the ol- factory bulb using double fluorescent labeling in GPR3 knockout/LacZ knock-in mice. Representative images of the olfactory bulb stained with SPiDER-βGal (A) and Nissl (B) in heterozygous GPR3 knockout/LacZ knock-in mice are shown. Double fluorescent labeling of GPR3 knockout/LacZ knock-in mice was per- formed using antibodies for EF-hand Ca2+-binding proteins and SPiDER-βGal (for detailed methods see Experimental procedures). Representative images from the double staining of GPR3 with calbindin (C), CCK (D), calretinin (E), and TH (F) are shown. The percentage of GPR3-expressing cells that also ex- pressed each marker in the olfactory bulb was calculated (G). Data represent the % expression of each marker out of the total number of GPR3-expressing neurons in the olfactory bulb. The actual number of cells in each region is also shown (double-positive cells/total number of GPR3-positive cells). Data are expressed as the mean ± SE (n = 3 mice/group). Scale bars = 100 µm.

Article Snippet: For immunohistochemistry, at least two sections obtained from each mouse were incubated overnight at 4 °C in the following primary antibodies, all diluted at 1:400 in PBS: anti-parvalbumin mouse monoclonal antibody (MAB1572, Merck, Darmstadt, Germany), anti-calretinin rabbit polyclonal antibody (HPA007305, Atlas Antibody, Bromma, Sweden), anti-calbindin mouse monoclonal antibody (C9848, Sigma Aldrich, St. Louis, MO), anti-GAD67 mouse monoclonal antibody (MAB5406, Merck), anti-NECAB2 rabbit polyclonal antibody (HPA013998, Atlas Antibody), anti-tyrosine hydroxylase rabbit polyclonal antibody (AB152, Sigma Aldrich), anti-DARPP32 goat polyclonal antibody (AF6259, R&D Systems, Minneapolis, MN), anti-somatostatin rabbit polyclonal antibody (HPA019472, Atlas Antibody), anti-β3-tubulin rabbit monoclonal antibody (D71G9, Cell Signaling Technology, Danvers, MA), anti-CTIP2 rat monoclonal antibody (25B6, Abcam, Cambridge, UK), anti-cholecystokinin rabbit polyclonal antibody (C2581, Sigma Aldrich), and anti-NeuN mouse monoclonal antibody (MAB377, Sigma Aldrich).

Techniques: Labeling, Knock-Out, Knock-In, Staining, Binding Assay, Double Staining, Expressing, Marker

Journal: Brain research

Article Title: Detailed neuronal distribution of GPR3 and its co-expression with EF-hand calcium-binding proteins in the mouse central nervous system.

doi: 10.1016/j.brainres.2020.147166

Figure Lengend Snippet: Fig. 5. Distribution and characterization of GPR3-positive neurons in the tha- lamus using double fluorescent labeling in GPR3 knockout/LacZ knock-in mice. Representative images of the thalamus stained with SPiDER-βGal (A) and Nissl (B) in heterozygous GPR3 knockout/LacZ knock-in mice are shown. Double fluorescent labeling of GPR3 knockout/LacZ knock-in mice was performed using antibodies for EF-hand Ca2+-binding proteins and SPiDER-βGal. Representative images from the thalamus with double staining of GPR3 with calretinin (C) and parvalbumin (D, E) are shown. The percentage of GPR3-ex- pressing cells that also expressed parvalbumin in the thalamus was calculated (F). Data represent the % expression of each marker out of the total number of GPR3-expressing neurons in the thalamus. The actual number of cells in each region is also shown (double-positive cells/total number of GPR3-positive cells). Data are expressed as the mean ± SE (n = 3 mice/group). Scale bars = 500 µm. (caption on next page)

Article Snippet: For immunohistochemistry, at least two sections obtained from each mouse were incubated overnight at 4 °C in the following primary antibodies, all diluted at 1:400 in PBS: anti-parvalbumin mouse monoclonal antibody (MAB1572, Merck, Darmstadt, Germany), anti-calretinin rabbit polyclonal antibody (HPA007305, Atlas Antibody, Bromma, Sweden), anti-calbindin mouse monoclonal antibody (C9848, Sigma Aldrich, St. Louis, MO), anti-GAD67 mouse monoclonal antibody (MAB5406, Merck), anti-NECAB2 rabbit polyclonal antibody (HPA013998, Atlas Antibody), anti-tyrosine hydroxylase rabbit polyclonal antibody (AB152, Sigma Aldrich), anti-DARPP32 goat polyclonal antibody (AF6259, R&D Systems, Minneapolis, MN), anti-somatostatin rabbit polyclonal antibody (HPA019472, Atlas Antibody), anti-β3-tubulin rabbit monoclonal antibody (D71G9, Cell Signaling Technology, Danvers, MA), anti-CTIP2 rat monoclonal antibody (25B6, Abcam, Cambridge, UK), anti-cholecystokinin rabbit polyclonal antibody (C2581, Sigma Aldrich), and anti-NeuN mouse monoclonal antibody (MAB377, Sigma Aldrich).

Techniques: Labeling, Knock-Out, Knock-In, Staining, Binding Assay, Double Staining, Expressing, Marker

Journal: Brain research

Article Title: Detailed neuronal distribution of GPR3 and its co-expression with EF-hand calcium-binding proteins in the mouse central nervous system.

doi: 10.1016/j.brainres.2020.147166

Figure Lengend Snippet: Fig. 7. Distribution and characterization of GPR3-positive neurons in the substantia nigra using double fluorescent labeling in GPR3 knockout/LacZ knock-in mice. Representative images of the substantia nigra stained with SPiDER-βGal (A) and Nissl (B) in heterozygous GPR3 knockout/LacZ knock-in mice. Double fluorescent labeling of GPR3 knockout/LacZ knock-in mice was performed using antibodies for EF-hand Ca2+-binding proteins and SPiDER-βGal. Representative images from double fluorescent labeling of GPR3 with TH (C), parvalbumin (D), calretinin (E), and calbindin (F) in the SNpr and TH (G) in the SNc are shown. Representative images from double fluorescent labeling of GPR3 with TH (H, I) in the VTA are shown. The fluorescent double-positive neurons are indicated by arrowheads. The percentage of GPR3-expressing cells that also expressed each marker in the SNpr was calculated (J). Data represent the % expression of each marker out of the total number of GPR3-expressing neurons in the SNpr. The actual number of cells in each region is also shown (double-positive cells/total number of GPR3-positive cells). Data are expressed as the mean ± SE (n = 3 mice/group). Scale bars = 500 µm (A, B, G, H), 100 µm (C–F).

Article Snippet: For immunohistochemistry, at least two sections obtained from each mouse were incubated overnight at 4 °C in the following primary antibodies, all diluted at 1:400 in PBS: anti-parvalbumin mouse monoclonal antibody (MAB1572, Merck, Darmstadt, Germany), anti-calretinin rabbit polyclonal antibody (HPA007305, Atlas Antibody, Bromma, Sweden), anti-calbindin mouse monoclonal antibody (C9848, Sigma Aldrich, St. Louis, MO), anti-GAD67 mouse monoclonal antibody (MAB5406, Merck), anti-NECAB2 rabbit polyclonal antibody (HPA013998, Atlas Antibody), anti-tyrosine hydroxylase rabbit polyclonal antibody (AB152, Sigma Aldrich), anti-DARPP32 goat polyclonal antibody (AF6259, R&D Systems, Minneapolis, MN), anti-somatostatin rabbit polyclonal antibody (HPA019472, Atlas Antibody), anti-β3-tubulin rabbit monoclonal antibody (D71G9, Cell Signaling Technology, Danvers, MA), anti-CTIP2 rat monoclonal antibody (25B6, Abcam, Cambridge, UK), anti-cholecystokinin rabbit polyclonal antibody (C2581, Sigma Aldrich), and anti-NeuN mouse monoclonal antibody (MAB377, Sigma Aldrich).

Techniques: Labeling, Knock-Out, Knock-In, Staining, Binding Assay, Expressing, Marker

Journal: Brain research

Article Title: Detailed neuronal distribution of GPR3 and its co-expression with EF-hand calcium-binding proteins in the mouse central nervous system.

doi: 10.1016/j.brainres.2020.147166

Figure Lengend Snippet: Fig. 9. Distribution and characterization of GPR3-positive neurons in the cer- ebellum using double fluorescent labeling in GPR3 knockout/LacZ knock-in mice. Representative images of the cerebellum stained with SPiDER-βGal (A) and Nissl (B) in heterozygous GPR3 knockout/LacZ knock-in mice. Double fluorescent labeling of GPR3 knockout/LacZ knock-in mice was performed using antibodies for EF-hand Ca2+-binding proteins and SPiDER-βGal. Representative images from double fluorescent labeling of GPR3 with calretinin (C) in the CGL of the cerebellum, and with calretinin (D) and parvalbumin (E) in the DCN are shown. The fluorescent double-positive neurons are indicated by arrowheads. The percentage of GPR3-expressing cells that also expressed each marker in the DCN of the cerebellum was calculated (F). Data represent the % expression of each marker out of the total number of GPR3-expressing neurons in the DCN of the cerebellum. The actual number of cells in each region is also shown (double-positive cells/total number of GPR3-positive cells). Data are expressed as the mean ± SE (n = 3 mice/group). Scale bars = 500 µm (A, B), 100 µm (C–E).

Article Snippet: For immunohistochemistry, at least two sections obtained from each mouse were incubated overnight at 4 °C in the following primary antibodies, all diluted at 1:400 in PBS: anti-parvalbumin mouse monoclonal antibody (MAB1572, Merck, Darmstadt, Germany), anti-calretinin rabbit polyclonal antibody (HPA007305, Atlas Antibody, Bromma, Sweden), anti-calbindin mouse monoclonal antibody (C9848, Sigma Aldrich, St. Louis, MO), anti-GAD67 mouse monoclonal antibody (MAB5406, Merck), anti-NECAB2 rabbit polyclonal antibody (HPA013998, Atlas Antibody), anti-tyrosine hydroxylase rabbit polyclonal antibody (AB152, Sigma Aldrich), anti-DARPP32 goat polyclonal antibody (AF6259, R&D Systems, Minneapolis, MN), anti-somatostatin rabbit polyclonal antibody (HPA019472, Atlas Antibody), anti-β3-tubulin rabbit monoclonal antibody (D71G9, Cell Signaling Technology, Danvers, MA), anti-CTIP2 rat monoclonal antibody (25B6, Abcam, Cambridge, UK), anti-cholecystokinin rabbit polyclonal antibody (C2581, Sigma Aldrich), and anti-NeuN mouse monoclonal antibody (MAB377, Sigma Aldrich).

Techniques: Labeling, Knock-Out, Knock-In, Staining, Binding Assay, Expressing, Marker

Journal: Brain research

Article Title: Detailed neuronal distribution of GPR3 and its co-expression with EF-hand calcium-binding proteins in the mouse central nervous system.

doi: 10.1016/j.brainres.2020.147166

Figure Lengend Snippet: Fig. 10. Distribution and characterization of GPR3-positive neurons in the spinal cord using double fluorescent labeling in GPR3 knockout/LacZ knock-in mice. Representative images of the spinal cord stained with SPiDER-βGal (A) and Nissl (B) in heterozygous GPR3 knockout/LacZ knock-in mice. Double fluorescent labeling of GPR3 knockout/LacZ knock-in mice was performed using antibodies for EF-hand Ca2+-binding proteins and SPiDER-βGal. Representative images from double fluorescent labeling of GPR3 with calretinin (C) and NECAB2 (D) in the AH of the spinal cord are shown. The fluorescent double-positive neurons are indicated by arrowheads. The percentage of GPR3- expressing cells that also expressed calretinin in the spinal cord was calculated (E). Data represent the % expression of calretinin out of the total number of GPR3-expressing neurons in the spinal cord. The actual number of cells in each region is also shown (double-positive cells/total number of GPR3-positive cells). Data are expressed as the mean ± SE (n = 3 mice/group). Scale bars = 500 µm (A, B), 100 µm (C, D).

Article Snippet: For immunohistochemistry, at least two sections obtained from each mouse were incubated overnight at 4 °C in the following primary antibodies, all diluted at 1:400 in PBS: anti-parvalbumin mouse monoclonal antibody (MAB1572, Merck, Darmstadt, Germany), anti-calretinin rabbit polyclonal antibody (HPA007305, Atlas Antibody, Bromma, Sweden), anti-calbindin mouse monoclonal antibody (C9848, Sigma Aldrich, St. Louis, MO), anti-GAD67 mouse monoclonal antibody (MAB5406, Merck), anti-NECAB2 rabbit polyclonal antibody (HPA013998, Atlas Antibody), anti-tyrosine hydroxylase rabbit polyclonal antibody (AB152, Sigma Aldrich), anti-DARPP32 goat polyclonal antibody (AF6259, R&D Systems, Minneapolis, MN), anti-somatostatin rabbit polyclonal antibody (HPA019472, Atlas Antibody), anti-β3-tubulin rabbit monoclonal antibody (D71G9, Cell Signaling Technology, Danvers, MA), anti-CTIP2 rat monoclonal antibody (25B6, Abcam, Cambridge, UK), anti-cholecystokinin rabbit polyclonal antibody (C2581, Sigma Aldrich), and anti-NeuN mouse monoclonal antibody (MAB377, Sigma Aldrich).

Techniques: Labeling, Knock-Out, Knock-In, Staining, Binding Assay, Expressing